Illumina Kits and Reagents for SARS-CoV-2 Whole Genome Sequencing Using MiSeq Systems

Summary (Newest Update)

Background The Mississippi State Department of Health (MSDH) aims to enhance its capabilities in Whole Genome Sequencing (WGS) to monitor and track SARS-CoV-2 mutations and variant strains within the Mississippi population. This initiative is critical for public health surveillance and outbreak detection, particularly in relation to foodborne illnesses through the PulseNet database, which connects cases using DNA sequencing. Work Details The contract involves the purchase of Illumina kits and reagents necessary for performing WGS on SARS-CoV-2 specimens using MiSeq sequencers. The specific products to be procured include: 1. Illumina® DNA Prep (M) Tagmentation (96 samples, IPB) - 30 cases at $3,257.00 each 2. Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) - 10 cases at $480.00 each 3. Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) - 10 cases at $480.00 each 4. Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) - 10 cases at $480.00 each 5. Illumina Microbial Amplicon Prep – Influenza A/B (48 samples) - 10 cases at $1,833.00 each 6. iSeq 100i1 Reagent v2 (300 cycle) 8 pack - 6 cases at $4,954.00 each 7. MiSeq Reagent Kit v2 (300 cycle) - 20 cases at $1,477.00 each 8. MiSeq Reagent Kit v2 (500 cycles per kit) - 80 cases at $1,661.00 each 9. NextSeq 1000/2000 P1 Reagents (300 Cycles) - 20 cases at $1,288.00 each 10. NextSeq 1000/2000 P1 Reagents (600 Cycles) v3 - 10 cases at $1,957.00 each. The total estimated cost for these kits and reagents is approximately $393,667.98 including freight and surcharges. Period of Performance The estimated cost for the kits and reagents covers a quoted period with pricing firm through February 12, 2027. Place of Performance The products will be delivered to the Mississippi Public Health Laboratory located in Jackson, Mississippi. Bidder Requirements Only Illumina is recognized as the sole manufacturer of the required kits and reagents for WGS on MiSeq sequencers; thus no other vendors can fulfill this requirement.