Protein Analysis Rainbow Trout

Project ID: 1232SA26Q0113 FederalOpportunitiesSynopsis Solicitation

Overview

Agency	Agricultural Research Service
Deadline	02/02/26
Posted	01/22/26
Estimated Value	Not Provided
Set Aside	Small Business
NAICS	541380 - Testing Laboratories and Services
PSC	B529 - Special Studies/Analysis- Scientific Data
Location	Kearneysville, WV 25430 United States

Description

Primary Latest Change

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation; offers are being requested, and a separate written solicitation will not be issued. Solicitation number 1232SA26Q0113 is issued as a Request for Quotation (RFQ) for Protein Analysis Rainbow Trout per the Statement of Work (Attachment 2). This acquisition is set-aside for small business concerns. This solicitation incorporates provisions and clauses by reference. The full text of provisions and clauses may be accessed electronically at www.acquisition.gov. The applicable North American Industry Classification Standard Code is 541380. The small business size standard is $19M. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency. See Attachment 1 - Solicitation Terms and Conditions for applicable clauses, instructions, and evaluation criteria.

Summary (Newest Update)

Background The USDA Agricultural Research Service (ARS) National Cold Water Aquaculture Research Facility (NCCCWA) is issuing this solicitation for the purpose of conducting protein analysis on rainbow trout. The goal of this contract is to obtain unbiased quantitative proteomics analysis to support research efforts in aquaculture. Work Details The contractor will perform 4D-DIA (data independent acquisition) on a LC-MS/MS platform for 36 lysosomal protein preparations isolated from rainbow trout liver. The work includes: 1. Sample preparation involving protein extraction, denaturation, reduction, tryptic digestion, and peptide cleanup. 2. Establishment of a spectral library using a pooled sample. 3. DIA data collection must be conducted using a timsTOF mass spectrometer (LC-MS/MS). Deliverables include a technical report detailing the experimental procedure, LC-MS/MS instrument parameters, and all identified and quantified data. The analysis should allow for pairwise comparisons between treatment groups and provide data in an Excel spreadsheet format within 60 days of sample receipt. Period of Performance The total duration of this contract shall not exceed 60 days from the date of award. Place of Performance USDA-NCCCWA, 11861 Leetown Rd, Kearneysville, WV 25430-3804

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