Low-Coverage Whole Genome Sequencing of Rainbow Trout Samples

Project ID: 1232SA26Q0416 FederalOpportunitiesSynopsis Solicitation

Overview

Agency	Agricultural Research Service
Deadline	05/26/26
Posted	05/14/26
Estimated Value	Not Provided
Set Aside	SBA
NAICS	541380 - Testing Laboratories and Services
PSC	Q301 - Reference Laboratory Testing
Location	Kearneysville, WV 25430 United States

Description

Primary Latest Change

Summary

This is a combined synopsis/solicitation for commercial service prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued. Solicitation number 1232SA26Q0416 is issued as a Request for Quotation (RFQ) for the USDA-ARS- NEA Cool and Cold Water Aquaculture, requiring genomic DNA extractions from tissue samples stored in 95% ethanol and 0.5x whole genome sequencing via Illumina technology of 2,000 rainbow trout samples (Genome size ~2.4Gb). The Contractor shall provide all services, supplies, equipment and facilities necessary to sequence and impute genotypes in accordance with the required performance elements and benchmarks. This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 The small business size standard $19. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency. See:

Attachment 2 Terms and Conditions

Attachment 3-Wage Determination-2015-5415-CO_Boulder Attachment 4-Wage Determination-2015-5613-CA_Los Angeles Attachment 5-Wage Determination-2015-5623-CA_Almeda-Contra Costa Attachment 6-Wage Determination-2015-5635-CA_San Diego Attachment 7-Wage Determination-2015-5859-ID_ Jerome-Twin Falls Attachment 8-Wage Determination-2015-6059-ME_Cumberland-Sagadahoc-York

Summary (Newest Update)

Background The USDA Agricultural Research Service (ARS) is issuing this solicitation to procure genomic DNA extractions and whole genome sequencing services for 2,000 rainbow trout samples. The goal of the contract is to support research in aquaculture by providing high-quality genomic data, which will aid in the understanding and improvement of fish breeding and health. This acquisition is specifically set aside for small business concerns. Work Details The contractor shall provide all services, supplies, equipment, and facilities necessary to perform the following tasks: 1. Conduct DNA extractions from fin tissues of 2,000 rainbow trout samples stored in 95% ethanol. 2. Perform low-coverage whole genome sequencing using Illumina technology with a minimum output of 1.2 Gbases per sample from quality-filtered paired-end reads. 3. Implement quality control measures throughout the process, including DNA quality checks using Qubit or similar instruments, library preparation with standard Illumina protocols, and documentation of QC procedures in the final project report. 4. Return all unused portions of tissue samples to the government upon completion of the project. Deliverables include DNA sequence files in FASTQ format, all unused tissue samples, and a comprehensive Final Project Report detailing DNA extraction processes, QC results, library preparations, sequencing outcomes, and summary data for each sample. Period of Performance June 01, 2026 – September 30, 2026 Place of Performance USDA-ARS-NEA Cool and Cold Water Aquaculture, 11861 Leetown Road, Kearneysville, WV 25430

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