DNA Extraction and Shotgun Metagenomics Sequencing of Fecal Samples

Project ID: NOI-CC-OLAO-26-00551 FederalOpportunitiesSpecial Notice

Overview

Agency	National Institutes of Health
Deadline	05/23/26
Posted	05/13/26
Estimated Value	Not Provided
Set Aside	None
NAICS	622110 - General Medical and Surgical Hospitals
PSC	Q301 - Reference Laboratory Testing
Location	Bethesda, MD 20892 United States

Description

Primary Latest Change

Summary

This is a Notice of Intent, not a request for proposal. The National Institute of Health intends to negotiate on an other than full and open competition basis with the following vendor: Rush University Medical Center.

Acquisition Description

This acquisition requested is for DNA extraction and shotgun metagenomics sequencing of fecal samples from humans (n=25) at baseline and after a ketogenic diet with/with NR supplementation (n=100 samples), and from mice (n=25) at baseline and after a high fat diet with/with NR supplementation (n=100 samples; total 200 samples). The results from these tests will be used to understand cross-species validation of mechanisms related to bile acid- and FXR-induced increases in ketogenesis. Services are required of (1) conducting shotgun metagenomics sequencing of fecal samples for 200 samples (including DNA extraction, library preparation and shotgun metagenomics sequencing) and (2) providing raw data files to the PI, Dr. Katherine Maki. The statutory authority for this sole source requirement is 41 U.S.C. 253(c) (1) as implemented by FAR 6.302-1 only one responsible source and no other supply of service will satisfy agency requirements. THIS IS NOT A REQUEST FOR PROPOSAL. All responsible sources that could provide the required may submit a capability statement that will be considered by email (subject line to reference NOI-CC-OLAO-26-005551) by 12:00 PM eastern standard time on 05/23/2026 to: valerie.gregorio@nih.gov. All responses received by the closing date of this synopsis will be considered by the Government. A determination not to compete this requirement, based upon responses to this notice, is solely within the discretion of the Government.

Summary (Newest Update)

Background The National Institutes of Health (NIH) intends to negotiate on a sole source basis with Rush University Medical Center for a contract focused on DNA extraction and shotgun metagenomics sequencing. This initiative aims to support research into the mechanisms related to bile acid and FXR-induced increases in ketogenesis, particularly through the analysis of fecal samples from humans and mice under specific dietary conditions. Work Details The contractor will be responsible for: (1) Conducting DNA extraction and shotgun metagenomics sequencing for a total of 200 fecal samples, which includes both human samples (n=100) collected at baseline and after a ketogenic diet with/without NR supplementation, and mouse samples (n=100) collected at baseline and after a high-fat diet with/without NR supplementation. (2) Providing raw data files to the Principal Investigator, Dr. Katherine Maki. The services include library preparation and sequencing as part of the shotgun metagenomics process. Place of Performance The work will be conducted at Rush University Medical Center located in Chicago, IL.

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